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KMID : 0381219750070030162
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Journal of RIMSK
1975 Volume.7 No. 3 p.162 ~ p.169
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Experimental studies of using a tissue protein of the epididymis
and seminal vesicle as an antifertility antigen
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Abstract
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Adult female white rabbits were used for the immunization. The rat epididymal and seminal vesical tissue were homogenized in a tissue grinder and centrifuged at 15,000 R.P.M. for 15 minutes. The supernatant was used as the antigen in this study. Included spermatozoa in the epididymis could not be excluded from this tissue but there were no sperms in the seminal vesicle. The antigen was diluted with saline (0. 15 M. NaCl) until a protein concentration of 10.0 mg/ml was reached.
The antigen was emulsified with an equal volume of complete Freund´s adjuvant at each time of immunization and 2. 0 ml were injected intradermally in the back of each rabbit. A similar injection was given 2 weeks later. After 2 more weeks each rabbit was given an injection of the antigen alone every other day three times. One week-after the last injection the rabbits were bled by heart puncture.
Experimental animals were divided into three groups. First group rabbit was treated with a tissue protein of epididymis. Second group rabbit was treated with a tissue protein of seminal vesicle. Third group rabbit was treated with an complex of epididymal and seminal vesical tissue protein.
The action of the antibodies on rabbit sperm and rat sperm was examined in sperm immobilization and sperm agglutination test with immunoelectrophoretic method. The rabbit antiserum produced by the first group of epididymal tissue protein was found to be capable of immobilizing and agglutinating rat sperm and rabbit sperm. The antiserum produced by the second group of seminal vesical tissue protein also showed the capacity to immobilize or
agglutinate sperm but the rabbit antiserum against complex antigen of epididymal tissue and seminal vesical tissue in third group was most significant on sperm immobilization and sperm agglutination test of rat and rabbit in those three groups which suggests one of the complex antigens would be an adjuvant action in antibody production.
An immunoelectrophoretic fractionation -of antigens revealed four major groups in the epididymal tissue protein, and one fraction in the seminal vesical tissue. Study is now in progress to evaluate the possibility of using those tissue proteins as antifertility antigens.
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